Differential expression of miRNAs in response to topping in flue-cured tobacco (Nicotiana tabacum) roots.

ABSTRACT:

Background

Topping is an important cultivating measure for flue-cured tobacco, and many genes had been found to be differentially expressed in response to topping. But it is still unclear how these genes are regulated. MiRNAs play a critical role in post-transcriptional gene regulation, so we sequenced two sRNA libraries from tobacco roots before and after topping, with a view to exploring transcriptional differences in miRNAs.

Methodology/principal findings

Two sRNA libraries were generated from tobacco roots before and after topping. Solexa high-throughput sequencing of tobacco small RNAs revealed a total of 12,104,207 and 11,292,018 reads representing 3,633,398 and 3,084,102 distinct sequences before and after topping. The expressions of 136 conserved miRNAs (belonging to

SUBMITTER: Guo H 

PROVIDER: S-EPMC3237444 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature