Tumor and salivary matrix metalloproteinase levels are strong diagnostic markers of oral squamous cell carcinoma.

ABSTRACT:

Background

The matrix metalloproteinases (MMP) cause degradation of the extracellular matrix and basement membranes, and thus may play a key role in cancer development.

Methods

In our search for biomarkers for oral squamous cell carcinomas (OSCC), we compared primary OSCC, oral dysplasia and control subjects with respect to: (i) expression of MMP1, MMP3, MMP10, and MMP12 in oral epithelial tissue using Affymetrix U133 2.0 Plus GeneChip arrays, followed by quantitative reverse transcription-PCR (qRT-PCR) for MMP1, and (ii) determination of MMP1 and MMP3 concentrations in saliva.

Results

MMP1 expression in primary OSCC (n = 119) was >200-fold higher (P = 7.16 × 10(-40)) compared with expression levels in nonneoplastic oral epithelium from controls (n = 35). qRT-PCR resu

SUBMITTER: Stott-Miller M 

PROVIDER: S-EPMC3237810 | biostudies-literature | 2011 Dec

REPOSITORIES: biostudies-literature