Physicochemical and biological characterization of 1E10 anti-idiotype vaccine.

ABSTRACT:

Background

1E10 monoclonal antibody is a murine anti-idiotypic antibody that mimics N-glycolyl-GM3 gangliosides. This antibody has been tested as an anti-idiotypic cancer vaccine, adjuvated in Al(OH)3, in several clinical trials for melanoma, breast, and lung cancer. During early clinical development this mAb was obtained in vivo from mice ascites fluid. Currently, the production process of 1E10 is being transferred from the in vivo to a bioreactor-based method.

Results

Here, we present a comprehensive molecular and immunological characterization of 1E10 produced by the two different production processes in order to determine the impact of the manufacturing process in vaccine performance. We observed differences in glycosylation pattern, charge heterogeneity and structural s

SUBMITTER: Machado YJ 

PROVIDER: S-EPMC3238287 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature