Protein and mineral composition of osteogenic extracellular matrix constructs generated with a flow perfusion bioreactor.

ABSTRACT: This study investigated the temporal composition of an osteogenic extracellular matrix construct generated by culturing mesenchymal stem cells in an electrospun biodegradable poly(ε-caprolactone) fiber mesh scaffold within a flow perfusion bioreactor. Constructs of different extracellular matrix maturities were analyzed for their protein and mineral composition at several culture durations by liquid chromatography-tandem mass spectrometry, scanning electron microscopy, energy-dispersive X-ray diffraction, X-ray diffraction, and calcium and phosphate assays. The analysis revealed that at short culture durations the cells deposited cellular adhesion proteins as a prerequisite protein network for further bone formation. At the later culture durations, the extracellular matrix was composed of

SUBMITTER: Thibault RA 

PROVIDER: S-EPMC3238915 | biostudies-literature | 2011 Dec

REPOSITORIES: biostudies-literature