The membrane fusion step of vaccinia virus entry is cooperatively mediated by multiple viral proteins and host cell components.

ABSTRACT: For many viruses, one or two proteins allow cell attachment and entry, which occurs through the plasma membrane or following endocytosis at low pH. In contrast, vaccinia virus (VACV) enters cells by both neutral and low pH routes; four proteins mediate cell attachment and twelve that are associated in a membrane complex and conserved in all poxviruses are dedicated to entry. The aim of the present study was to determine the roles of cellular and viral proteins in initial stages of entry, specifically fusion of the membranes of the mature virion and cell. For analysis of the role of cellular components, we used well characterized inhibitors and measured binding of a recombinant VACV virion containing Gaussia luciferase fused to a core protein; viral and cellular membrane lipid mixing with a

SUBMITTER: Laliberte JP 

PROVIDER: S-EPMC3240603 | biostudies-literature | 2011 Dec

REPOSITORIES: biostudies-literature