Deep sequencing whole transcriptome exploration of the σE regulon in Neisseria meningitidis.

ABSTRACT: Bacteria live in an ever-changing environment and must alter protein expression promptly to adapt to these changes and survive. Specific response genes that are regulated by a subset of alternative σ(70)-like transcription factors have evolved in order to respond to this changing environment. Recently, we have described the existence of a σ(E) regulon including the anti-σ-factor MseR in the obligate human bacterial pathogen Neisseria meningitidis. To unravel the complete σ(E) regulon in N. meningitidis, we sequenced total RNA transcriptional content of wild type meningococci and compared it with that of mseR mutant cells (ΔmseR) in which σ(E) is highly expressed. Eleven coding genes and one non-coding gene were found to be differentially expressed between H44/76 wildtype and H44/76ΔmseR ce

SUBMITTER: Huis in 't Veld RA 

PROVIDER: S-EPMC3240639 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature