Protein composition of interband regions in polytene and cell line chromosomes of Drosophila melanogaster.

ABSTRACT:

Background

Despite many efforts, little is known about distribution and interactions of chromatin proteins which contribute to the specificity of chromomeric organization of interphase chromosomes. To address this issue, we used publicly available datasets from several recent Drosophila genome-wide mapping and annotation projects, in particular, those from modENCODE project, and compared molecular organization of 13 interband regions which were accurately mapped previously.

Results

Here we demonstrate that in interphase chromosomes of Drosophila cell lines, the interband regions are enriched for a specific set of proteins generally characteristic of the "open" chromatin (RNA polymerase II, CHRIZ (CHRO), BEAF-32, BRE1, dMI-2, GAF, NURF301, WDS and TRX). These regions also dis

SUBMITTER: Demakov SA 

PROVIDER: S-EPMC3240664 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature