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Testing pollen of single and stacked insect-resistant Bt-maize on in vitro reared honey bee larvae.


ABSTRACT: The ecologically and economic important honey bee (Apis mellifera) is a key non-target arthropod species in environmental risk assessment (ERA) of genetically modified (GM) crops. Honey bee larvae are directly exposed to transgenic products by the consumption of GM pollen. But most ERA studies only consider responses of adult bees, although Bt-proteins primarily affect the larval phases of target organisms. We adopted an in vitro larvae rearing system, to assess lethal and sublethal effects of Bt-pollen consumption in a standardized eco-toxicological bioassay. The effects of pollen from two Bt-maize cultivars, one expressing a single and the other a total of three Bt-proteins, on the survival and prepupae weight of honey bee larvae were analyzed. The control treatments included pollen from

SUBMITTER: Hendriksma HP 

PROVIDER: S-EPMC3241620 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature