Quantitative field testing Rotylenchulus reniformis DNA from metagenomic samples isolated directly from soil.

ABSTRACT: A quantitative PCR procedure targeting the β-tubulin gene determined the number of Rotylenchulus reniformis Linford & Oliveira 1940 in metagenomic DNA samples isolated from soil. Of note, this outcome was in the presence of other soil-dwelling plant parasitic nematodes including its sister genus Helicotylenchus Steiner, 1945. The methodology provides a framework for molecular diagnostics of nematodes from metagenomic DNA isolated directly from soil.

SUBMITTER: Showmaker K 

PROVIDER: S-EPMC3241691 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature