Crucial role of CD40 signaling in vascular wall cells in neointimal formation and vascular remodeling after vascular interventions.

ABSTRACT:

Objective

It has been shown that CD40-TRAF6 axis in leukocytes plays a significant role in neointimal formation after carotid ligation. Because CD40 and TRAF6 are expressed not only in leukocytes but also in vascular cells, we examined the role of CD40 contributed by vascular wall cells in neointimal formation after carotid ligation in an atherogenic environment.

Methods and results

Both CD40 and TRAF6 in medial smooth muscle cells (SMCs) was upregulated significantly at 3 days and more prominently at 7 days after injury in wildtype mice, but the TRAF6 upregulation was abolished in CD40(-/-) mice. In vitro, TRAF6 expression was induced by cytokines (tumor necrosis factor -α, interleukin-1β) via a NF-κB-dependent manner in wildtype SMCs, but this induction was blocked in CD40

SUBMITTER: Song Z 

PROVIDER: S-EPMC3241889 | biostudies-literature | 2012 Jan

REPOSITORIES: biostudies-literature