Cortactin colocalizes with filopodial actin and accumulates at IgCAM adhesion sites in Aplysia growth cones.

ABSTRACT: Both IgCAMs and the actin cytoskeleton play critical roles in neuronal growth cone motility and guidance. However, it is unclear how IgCAM receptors transduce signals from the plasma membrane to induce actin remodeling. Previous studies have shown that local clustering and immobilization of apCAM, the Aplysia homolog of NCAM, induces Src kinase activity and F-actin polymerization in the peripheral domain of cultured Aplysia bag cell growth cones. Therefore, we wanted to test whether the Src kinase substrate and actin regulator cortactin could be a molecular link between Src activity and actin assembly during apCAM-mediated growth cone guidance. Here, we cloned Aplysia cortactin and showed that it is abundant in the nervous system. Immunostaining of growth cones revealed a strong colocaliza

SUBMITTER: Decourt B 

PROVIDER: S-EPMC3241979 | biostudies-literature | 2009 Apr

REPOSITORIES: biostudies-literature