Asymmetric proteome equalization of the skeletal muscle proteome using a combinatorial hexapeptide library.

ABSTRACT: Immobilized combinatorial peptide libraries have been advocated as a strategy for equalization of the dynamic range of a typical proteome. The technology has been applied predominantly to blood plasma and other biological fluids such as urine, but has not been used extensively to address the issue of dynamic range in tissue samples. Here, we have applied the combinatorial library approach to the equalization of a tissue where there is also a dramatic asymmetry in the range of abundances of proteins; namely, the soluble fraction of skeletal muscle. We have applied QconCAT and label-free methodology to the quantification of the proteins that bind to the beads as the loading is progressively increased. Although some equalization is achieved, and the most abundant proteins no longer dominate t

SUBMITTER: Rivers J 

PROVIDER: S-EPMC3242751 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature