Site-directed mutagenesis reveals key residue for O antigen chain length regulation and protein stability in Pseudomonas aeruginosa Wzz2.

ABSTRACT: The production of preferred lipopolysaccharide O antigen chain lengths is important for the survival of pathogenic Gram-negative bacteria in different environments, yet how Wzz proteins regulate these lengths is not well understood. The Wzz2 proteins from two different serotype O11 Pseudomonas aeruginosa strains are responsible for the expression of different very long chain lengths despite high sequence homology. Site-directed mutagenesis was performed to determine whether a specific amino acid was responsible for this difference in chain length; the residue present in position 321 within the second predicted coiled-coil region was able to determine which chain length was produced. A panel of site-directed mutants introducing different amino acids at this position implicated that the char

SUBMITTER: Kintz EN 

PROVIDER: S-EPMC3243511 | biostudies-literature | 2011 Dec

REPOSITORIES: biostudies-literature