Left-to-right ventricular differences in I(KATP) underlie epicardial repolarization gradient during global ischemia.

ABSTRACT:

Background

The ionic mechanisms of electrical heterogeneity in the ischemic ventricular epicardium remain poorly understood.

Objective

This study sought to test the hypothesis that the adenosine triphosphate (ATP)-activated K+ current (I(KATP)) plays an important role in mediating repolarization differences between the right ventricle (RV) and left ventricle (LV) during global ischemia.

Methods

Electrical activity in Langendorff-perfused guinea pig hearts was recorded optically during control, ischemia, and reperfusion. Patch-clamp experiments were used to quantify I(KATP) density in isolated myocytes. Molecular correlates of I(KATP) (Kir6/SUR) were probed via reverse transcriptase-polymerase chain reaction. The role of I(KATP) in modulating repolarization was studied

SUBMITTER: Pandit SV 

PROVIDER: S-EPMC3244837 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature