Imaging single endocytic events reveals diversity in clathrin, dynamin and vesicle dynamics.

ABSTRACT: The dynamics of clathrin-mediated endocytosis can be assayed using fluorescently tagged proteins and total internal reflection fluorescence microscopy. Many of these proteins, including clathrin and dynamin, are soluble and changes in fluorescence intensity can be attributed either to membrane/vesicle movement or to changes in the numbers of individual molecules. It is important for assays to discriminate between physical membrane events and the dynamics of molecules. Two physical events in endocytosis were investigated: vesicle scission from the plasma membrane and vesicle internalization. Single vesicle analysis allowed the characterization of dynamin and clathrin dynamics relative to scission and internalization. We show that vesicles remain proximal to the plasma membrane for variable

SUBMITTER: Mattheyses AL 

PROVIDER: S-EPMC3245319 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature