High-throughput screening for inhibitors of sialyl- and fucosyltransferases.

ABSTRACT: Sweet screens: A high-throughput screening platform for identification of inhibitors of sialyl- and fucosyltransferases based on fluorescence polarization (FP) has been developed. An analogue of the natural donor substrate carrying a fluorescent label (green star) is transferred to a glycoprotein acceptor, which results in robust FP. The screening of 16,000 compounds against different glycosyltransferases has identified various interesting inhibitors.

SUBMITTER: Rillahan CD 

PROVIDER: S-EPMC3245354 | biostudies-literature | 2011 Dec

REPOSITORIES: biostudies-literature