A phospho-proteomic screen identifies substrates of the checkpoint kinase Chk1.

ABSTRACT:

Background

The cell-cycle checkpoint kinase Chk1 is essential in mammalian cells due to its roles in controlling processes such as DNA replication, mitosis and DNA-damage responses. Despite its paramount importance, how Chk1 controls these functions remains unclear, mainly because very few Chk1 substrates have hitherto been identified.

Results

Here, we combine a chemical genetics approach with high-resolution mass spectrometry to identify novel Chk1 substrates and their phosphorylation sites. The list of targets produced reveals the potential impact of Chk1 function not only on processes where Chk1 was already known to be involved, but also on other key cellular events such as transcription, RNA splicing and cell fate determination. In addition, we validate and explore the p

SUBMITTER: Blasius M 

PROVIDER: S-EPMC3245618 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature