Dif1 is a DNA-damage-regulated facilitator of nuclear import for ribonucleotide reductase.

ABSTRACT: The control of dNTP concentrations is critical to the fidelity of DNA synthesis and repair. One level of regulation is through subcellular localization of ribonucleotide reductase. In Saccharomyces cerevisiae, the small subunit Rnr2-Rnr4 is nuclear, whereas the large subunit Rnr1 is cytoplasmic. In response to S phase or DNA damage, Rnr2-Rnr4 enters the cytoplasm to bind Rnr1, forming an active complex. We previously reported that Wtm1 anchors Rnr2-Rnr4 in the nucleus. Here, we identify DIF1, which regulates localization of Rnr2-Rnr4. Dif1 binds directly to the Rnr2-Rnr4 complex through a conserved Hug domain to drive nuclear import. Dif1 is both cell-cycle and DNA-damage regulated, the latter of which occurs via the Mec1-Dun1 pathway. In response to DNA damage, Dun1 directly phosphorylate

SUBMITTER: Lee YD 

PROVIDER: S-EPMC3245869 | biostudies-literature | 2008 Oct

REPOSITORIES: biostudies-literature