La enhances IRES-mediated translation of laminin B1 during malignant epithelial to mesenchymal transition.

ABSTRACT: The majority of transcripts that harbor an internal ribosome entry site (IRES) are involved in cancer development via corresponding proteins. A crucial event in tumor progression referred to as epithelial to mesenchymal transition (EMT) allows carcinoma cells to acquire invasive properties. The translational activation of the extracellular matrix component laminin B1 (LamB1) during EMT has been recently reported suggesting an IRES-mediated mechanism. In this study, the IRES activity of LamB1 was determined by independent bicistronic reporter assays. Strong evidences exclude an impact of cryptic promoter or splice sites on IRES-driven translation of LamB1. Furthermore, no other LamB1 mRNA species arising from alternative transcription start sites or polyadenylation signals were detected tha

SUBMITTER: Petz M 

PROVIDER: S-EPMC3245933 | biostudies-literature | 2012 Jan

REPOSITORIES: biostudies-literature