Histone hypoacetylation is required to maintain late replication timing of constitutive heterochromatin.

ABSTRACT: The replication of the genome is a spatio-temporally highly organized process. Yet, its flexibility throughout development suggests that this process is not genetically regulated. However, the mechanisms and chromatin modifications controlling replication timing are still unclear. We made use of the prominent structure and defined heterochromatic landscape of pericentric regions as an example of late replicating constitutive heterochromatin. We manipulated the major chromatin markers of these regions, namely histone acetylation, DNA and histone methylation, as well as chromatin condensation and determined the effects of these altered chromatin states on replication timing. Here, we show that manipulation of DNA and histone methylation as well as acetylation levels caused large-scale hetero

SUBMITTER: Casas-Delucchi CS 

PROVIDER: S-EPMC3245938 | biostudies-literature | 2012 Jan

REPOSITORIES: biostudies-literature