Lentivirus transduction of human osteoclast precursor cells and differentiation into functional osteoclasts.

ABSTRACT: Gene transfer into stem cells has been an ongoing priority as a treatment for genetic disease and cancer for more than two decades. Methods described herein, form the basis for providing the cell source to determine if osteoclast precursor cells (OcP) can be used as therapeutic gene delivery systems in vivo. Osteoclasts and tumor associated macrophages or OcP, support survival, tumor progression and osteolysis in bone cancers. Two sources of precursor cells are compared: CD14+ cells, the standard OcP, found abundantly in peripheral blood and CD34+ cells, hematopoietic stem cells that are rare, but which can be expanded into OcP. Our findings characterize cell yield at each step of the transduction process and thus provide essential data for planning future in vivo experiments. In addition

SUBMITTER: Ramnaraine ML 

PROVIDER: S-EPMC3246560 | biostudies-literature | 2012 Jan

REPOSITORIES: biostudies-literature