Connecting rare DNA conformations and surface dynamics using single-molecule resonance energy transfer.

ABSTRACT: A mechanistic understanding of single-stranded DNA (ssDNA) behavior in the near-surface environment is critical to advancing DNA-directed self-assembled nanomaterials. A new approach is described that uses total internal reflection fluorescence microscopy to measure resonance energy transfer at the single-molecule level, providing a mechanistic understanding of the connection between molecular conformation and interfacial dynamics near amine-modified surfaces. Large numbers (>10(5)) of ssDNA trajectories were observed, permitting dynamic correlation of molecular conformation with desorption and surface mobility. On the basis of dynamic behavior, molecules could be designated as members of the more common coiled population or a rare, weakly bound conformation. Molecules in the coiled state

SUBMITTER: Kastantin M 

PROVIDER: S-EPMC3246573 | biostudies-literature | 2011 Dec

REPOSITORIES: biostudies-literature