Antibodies against the cardiac sodium/bicarbonate co-transporter (NBCe1) as pharmacological tools.

ABSTRACT:

Background and purpose

Na(+) /HCO(3) (-) co-transport (NBC) regulates intracellular pH (pH(i) ) in the heart. We have studied the electrogenic NBC isoform NBCe1 by examining the effect of functional antibodies to this protein.

Experimental approach

We generated two antibodies against putative extracellular loop domains 3 (a-L3) and 4 (a-L4) of NBCe1 which recognized NBCe1 on immunoblots and immunostaining experiments. pH(i) was monitored using epi-fluorescence measurements in cat ventricular myocytes. Transport activity of total NBC and of NBCe1 in isolation were evaluated after an ammonium ion-induced acidosis (expressed as H(+) flux, J(H) , in mmol·L(-1)  min(-1) at pH(i) 6.8) and during membrane depolarization with high extracellular potassium (potassium pulse, expressed

SUBMITTER: De Giusti VC 

PROVIDER: S-EPMC3246661 | biostudies-literature | 2011 Dec

REPOSITORIES: biostudies-literature