Rapid in vivo analysis of synthetic promoters for plant pathogen phytosensing.

ABSTRACT:

Background

We aimed to engineer transgenic plants for the purpose of early detection of plant pathogen infection, which was accomplished by employing synthetic pathogen inducible promoters fused to reporter genes for altered phenotypes in response to the pathogen infection. Toward this end, a number of synthetic promoters consisting of inducible regulatory elements fused to a red fluorescent protein (RFP) reporter were constructed for use in phytosensing.

Results

For rapid analysis, an Agrobacterium-mediated transient expression assay was evaluated, then utilized to assess the inducibility of each synthetic promoter construct in vivo. Tobacco (Nicotiana tabacum cv. Xanthi) leaves were infiltrated with Agrobacterium harboring the individual synthetic promoter-reporter constru

SUBMITTER: Liu W 

PROVIDER: S-EPMC3247077 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature