An alternative physiological role for the EmhABC efflux pump in Pseudomonas fluorescens cLP6a.

ABSTRACT:

Background

Efflux pumps belonging to the resistance-nodulation-division (RND) superfamily in bacteria are involved in antibiotic resistance and solvent tolerance but have an unknown physiological role. EmhABC, a RND-type efflux pump in Pseudomonas fluorescens strain cLP6a, extrudes hydrophobic antibiotics, dyes and polycyclic aromatic hydrocarbons including phenanthrene. The effects of physico-chemical factors such as temperature or antibiotics on the activity and expression of EmhABC were determined in order to deduce its physiological role(s) in strain cLP6a in comparison to the emhB disruptant strain, cLP6a-1.

Results

Efflux assays conducted with (14)C-phenanthrene showed that EmhABC activity is affected by incubation temperature. Increased phenanthrene efflux was measure

SUBMITTER: Adebusuyi AA 

PROVIDER: S-EPMC3247133 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature