Microplate-based platform for combined chromatin and DNA methylation immunoprecipitation assays.

ABSTRACT:

Background

The processes that compose expression of a given gene are far more complex than previously thought presenting unprecedented conceptual and mechanistic challenges that require development of new tools. Chromatin structure, which is regulated by DNA methylation and histone modification, is at the center of gene regulation. Immunoprecipitations of chromatin (ChIP) and methylated DNA (MeDIP) represent a major achievement in this area that allow researchers to probe chromatin modifications as well as specific protein-DNA interactions in vivo and to estimate the density of proteins at specific sites genome-wide. Although a critical component of chromatin structure, DNA methylation has often been studied independently of other chromatin events and transcription.

Results

SUBMITTER: Yu J 

PROVIDER: S-EPMC3247195 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature