Pax3 stimulates p53 ubiquitination and degradation independent of transcription.

ABSTRACT:

Background

Pax3 is a developmental transcription factor that is required for neural tube and neural crest development. We previously showed that inactivating the p53 tumor suppressor protein prevents neural tube and cardiac neural crest defects in Pax3-mutant mouse embryos. This demonstrates that Pax3 regulates these processes by blocking p53 function. Here we investigated the mechanism by which Pax3 blocks p53 function.

Methodology/principal findings

We employed murine embryonic stem cell (ESC)-derived neuronal precursors as a cell culture model of embryonic neuroepithelium or neural crest. Pax3 reduced p53 protein stability, but had no effect on p53 mRNA levels or the rate of p53 synthesis. Full length Pax3 as well as fragments that contained either the DNA-binding paired

SUBMITTER: Wang XD 

PROVIDER: S-EPMC3247257 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature