The HOPE fixation technique--a promising alternative to common prostate cancer biobanking approaches.

ABSTRACT:

Background

The availability of well-annotated prostate tissue samples through biobanks is key for research. Whereas fresh-frozen tissue is well suited for a broad spectrum of molecular analyses, its storage and handling is complex and cost-intensive. Formalin-fixed paraffin-embedded specimens (FFPE) are easy to handle and economic to store, but their applicability for molecular methods is restricted. The recently introduced Hepes-glutamic acid-buffer mediated Organic solvent Protection Effect (HOPE) is a promising alternative, which might have the potential to unite the benefits of FFPE and fresh-frozen specimen. Aim of the study was to compare HOPE-fixed, FFPE and fresh-frozen bio-specimens for their accessibility for diagnostic and research purposes.

Methods

10 prostate ca

SUBMITTER: Braun M 

PROVIDER: S-EPMC3248383 | biostudies-literature | 2011 Dec

REPOSITORIES: biostudies-literature