Recovering protein-protein and domain-domain interactions from aggregation of IP-MS proteomics of coregulator complexes.

ABSTRACT: Coregulator proteins (CoRegs) are part of multi-protein complexes that transiently assemble with transcription factors and chromatin modifiers to regulate gene expression. In this study we analyzed data from 3,290 immuno-precipitations (IP) followed by mass spectrometry (MS) applied to human cell lines aimed at identifying CoRegs complexes. Using the semi-quantitative spectral counts, we scored binary protein-protein and domain-domain associations with several equations. Unlike previous applications, our methods scored prey-prey protein-protein interactions regardless of the baits used. We also predicted domain-domain interactions underlying predicted protein-protein interactions. The quality of predicted protein-protein and domain-domain interactions was evaluated using known binary inter

SUBMITTER: Mazloom AR 

PROVIDER: S-EPMC3248428 | biostudies-literature | 2011 Dec

REPOSITORIES: biostudies-literature