Decoding cell lineage from acquired mutations using arbitrary deep sequencing.

ABSTRACT: Because mutations are inevitable, the genome of each cell in a multicellular organism becomes unique and therefore encodes a record of its ancestry. Here we coupled arbitrary single primer PCR with next-generation DNA sequencing to catalog mutations and deconvolve the phylogeny of cultured mouse cells. This study helps pave the way toward construction of retrospective cell-fate maps based on mutations accumulating in genomes of somatic cells.

SUBMITTER: Carlson CA 

PROVIDER: S-EPMC3248619 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature