An efficient procedure for plant organellar genome assembly, based on whole genome data from the 454 GS FLX sequencing platform.

ABSTRACT:

Motivation

Complete organellar genome sequences (chloroplasts and mitochondria) provide valuable resources and information for studying plant molecular ecology and evolution. As high-throughput sequencing technology advances, it becomes the norm that a shotgun approach is used to obtain complete genome sequences. Therefore, to assemble organellar sequences from the whole genome, shotgun reads are inevitable. However, associated techniques are often cumbersome, time-consuming, and difficult, because true organellar DNA is difficult to separate efficiently from nuclear copies, which have been transferred to the nucleus through the course of evolution.

Results

We report a new, rapid procedure for plant chloroplast and mitochondrial genome sequencing and assembly using the Roche

SUBMITTER: Zhang T 

PROVIDER: S-EPMC3248859 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature