Development of a fritless packed column for capillary electrochromatography-mass spectrometry.

ABSTRACT: A novel procedure was developed for the fabrication of a fritless packed column for the coupling of capillary electrochromatography (CEC) to mass spectrometry (MS). The process involved the formation of internal tapers on two separate columns. Once the internal tapers are formed and the columns are packed, the untapered ends of each column were joined together by a commercially available connector. Several advantages of the fritless columns are described. First, the design used here eventually eliminates the need for any frits thus reducing the possibility of bubble formation seen with fritted packed columns. In addition, this is the first report in which the internal tapers are formed at both the inlet and outlet column ends making the fritless CEC-MS column more robust compared to only o

SUBMITTER: Bragg W 

PROVIDER: S-EPMC3249243 | biostudies-literature | 2011 Dec

REPOSITORIES: biostudies-literature