Yeast-like chronological senescence in mammalian cells: phenomenon, mechanism and pharmacological suppression.

ABSTRACT: In yeast, chronological senescence (CS) is defined as loss of viability in stationary culture. Although its relevance to the organismal aging remained unclear, yeast CS was one of the most fruitful models in aging research. Here we described a mammalian replica of yeast CS: loss of viability of overgrown "yellow" cancer cell culture. In a density and time (chronological)-dependent manner, cell culture loses the ability to re-grow in fresh medium. Rapamycin dramatically decelerated CS. Loss of viability was caused by acidification of the medium by lactic acid (lactate). Rapamycin decreased production of lactate, making conditioned medium (CM) less deadly. Both deadly CM and lactate caused loss of viability in low cell density, not preventable by either rapamycin or additional glucose. Also,

SUBMITTER: Leontieva OV 

PROVIDER: S-EPMC3249453 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature