An antibiotic selection marker for schistosome transgenesis.

ABSTRACT: Drug selection is widely used in transgene studies of microbial pathogens, mammalian cell and plant cell lines. Drug selection of transgenic schistosomes would be desirable to provide a means to enrich for populations of transgenic worms. We adapted murine leukaemia retrovirus vectors - widely used in human gene therapy research - to transduce schistosomes, leading to integration of transgenes into the genome of the blood fluke. A dose-response kill curve and lethal G418 (geneticin) concentrations were established: 125-1,000μg/ml G418 were progressively more toxic for schistosomules of Schistosoma mansoni with toxicity increasing with antibiotic concentration and with duration of exposure. By day 6 of exposure to ⩾500μg/ml, significantly fewer worms survived compared with non-exposed contr

SUBMITTER: Rinaldi G 

PROVIDER: S-EPMC3249473 | biostudies-literature | 2012 Jan

REPOSITORIES: biostudies-literature