Effects of internal low-dose irradiation from 131I on gene expression in normal tissues in Balb/c mice.

ABSTRACT:

Background

The aim of this study was to investigate the global gene expression response of normal tissues following internal low absorbed dose irradiation of 131I.

Methods

Balb/c mice were intravenously injected with 13 to 260 kBq of 131I and euthanized 24 h after injection. Kidneys, liver, lungs, and spleen were surgically removed. The absorbed dose to the tissues was 0.1 to 9.7 mGy. Total RNA was extracted, and Illumina MouseRef-8 Whole-Genome Expression BeadChips (Illumina, Inc., San Diego, California, USA) were used to compare the gene expression of the irradiated tissues to that of non-irradiated controls. The Benjamini-Hochberg method was used to determine differentially expressed transcripts and control for false discovery rate. Only transcripts with a modulation of 1

SUBMITTER: Schuler E 

PROVIDER: S-EPMC3251037 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature