Regulation of myotube formation by the actin-binding factor drebrin.

ABSTRACT:

Background

Myogenic differentiation involves cell-cycle arrest, activation of the muscle-specific transcriptome, and elongation, alignment and fusion of myoblasts into multinucleated myotubes. This process is controlled by promyogenic transcription factors and regulated by signaling pathways in response to extracellular cues. The p38 mitogen-activated protein kinase (p38 MAPK) pathway promotes the activity of several such transcription factors, including MyoD and MEF2, thereby controlling the muscle-specific transcription program. However, few p38-regulated genes that play a role in the regulation of myogenesis have been identified.

Methods

RNA interference (RNAi), chemical inhibition and immunofluorescence approaches were used to assess the role of drebrin in differentiatio

SUBMITTER: Mancini A 

PROVIDER: S-EPMC3251523 | biostudies-literature | 2011 Dec

REPOSITORIES: biostudies-literature