High Resolution Melt (HRM) analysis is an efficient tool to genotype EMS mutants in complex crop genomes.

ABSTRACT:

Background

Targeted Induced Loci Lesions IN Genomes (TILLING) is increasingly being used to generate and identify mutations in target genes of crop genomes. TILLING populations of several thousand lines have been generated in a number of crop species including Brassica rapa. Genetic analysis of mutants identified by TILLING requires an efficient, high-throughput and cost effective genotyping method to track the mutations through numerous generations. High resolution melt (HRM) analysis has been used in a number of systems to identify single nucleotide polymorphisms (SNPs) and insertion/deletions (IN/DELs) enabling the genotyping of different types of samples. HRM is ideally suited to high-throughput genotyping of multiple TILLING mutants in complex crop genomes. To date it has been

SUBMITTER: Lochlainn SO 

PROVIDER: S-EPMC3251530 | biostudies-literature | 2011 Dec

REPOSITORIES: biostudies-literature