New microsatellite markers developed from Urochloa humidicola (Poaceae) and cross amplification in different Urochloa species.

ABSTRACT:

Background

Urochloa humidicola is a forage grass that grows in tropical regions and is recognized for its tolerance to seasonal flooding. It is a polyploid and apomictic species with high phenotypic plasticity. As molecular tools are important in facilitating the development of new cultivars and in the classification of related species, the objectives of this study were to develop new polymorphic microsatellite markers from an enriched library constructed from U. humidicola and to evaluate their transferability to other Urochloa species.

Findings

Microsatellite sequences were identified from a previously constructed enriched library, and specific primers were designed for 40 loci. Isolated di-nucleotide repeat motifs were the most abundant followed by tetra-nucleotide repeat

SUBMITTER: Vigna BB 

PROVIDER: S-EPMC3251623 | biostudies-literature | 2011 Dec

REPOSITORIES: biostudies-literature