Elevated mRNA expression of CHAC1 splicing variants is associated with poor outcome for breast and ovarian cancer patients.

ABSTRACT:

Background

The role of CHAC1 (cation transport regulator-like protein 1), a recently identified component of the unfolded protein response (UPR) pathway, in gynaecological cancers has not yet been characterised. Now, this work illustrates CHAC1 mRNA expression and associated clinical outcome in breast and ovarian cancer.

Methods

The prognostic value of CHAC1 and its two transcript variants was investigated in 116 breast and 133 ovarian tissues using quantitative real-time reverse-transcriptase PCR. Subsequently, we conducted functional studies using short-interfering RNA-mediated knockdown and plasmid-mediated overexpression of CHAC1 in breast and ovarian cancer cells.

Results

Poorly differentiated tumours exhibited higher CHAC1 mRNA expression (breast cancer: P=0.004

SUBMITTER: Goebel G 

PROVIDER: S-EPMC3251857 | biostudies-literature | 2012 Jan

REPOSITORIES: biostudies-literature