Combined blood/tissue analysis for cancer biomarker discovery: application to renal cell carcinoma.

ABSTRACT: A method that relies on subtractive tissue-directed shot-gun proteomics to identify tumor proteins in the blood of a patient newly diagnosed with cancer is described. To avoid analytical and statistical biases caused by physiologic variability of protein expression in the human population, this method was applied on clinical specimens obtained from a single patient diagnosed with nonmetastatic renal cell carcinoma (RCC). The proteomes extracted from tumor, normal adjacent tissue and preoperative plasma were analyzed using 2D-liquid chromatography-mass spectrometry (LC-MS). The lists of identified proteins were filtered to discover proteins that (i) were found in the tumor but not normal tissue, (ii) were identified in matching plasma, and (iii) whose spectral count was higher in tumor tiss

SUBMITTER: Johann DJ 

PROVIDER: S-EPMC3251958 | biostudies-literature | 2010 Mar

REPOSITORIES: biostudies-literature