Quantitative proteomics analysis of adsorbed plasma proteins classifies nanoparticles with different surface properties and size.

ABSTRACT: Nanoparticle biological activity, biocompatibility and fate can be directly affected by layers of readily adsorbed host proteins in biofluids. Here, we report a study on the interactions between human blood plasma proteins and nanoparticles with a controlled systematic variation of properties using (18)O-labeling and LC-MS-based quantitative proteomics. We developed a novel protocol to both simplify isolation of nanoparticle bound proteins and improve reproducibility. LC-MS analysis identified and quantified 88 human plasma proteins associated with polystyrene nanoparticles consisting of three different surface chemistries and two sizes, as well as, for four different exposure times (for a total of 24 different samples). Quantitative comparison of relative protein abundances was achieved b

SUBMITTER: Zhang H 

PROVIDER: S-EPMC3252235 | biostudies-literature | 2011 Dec

REPOSITORIES: biostudies-literature