The metabolome of induced pluripotent stem cells reveals metabolic changes occurring in somatic cell reprogramming.

ABSTRACT: Metabolism is vital to every aspect of cell function, yet the metabolome of induced pluripotent stem cells (iPSCs) remains largely unexplored. Here we report, using an untargeted metabolomics approach, that human iPSCs share a pluripotent metabolomic signature with embryonic stem cells (ESCs) that is distinct from their parental cells, and that is characterized by changes in metabolites involved in cellular respiration. Examination of cellular bioenergetics corroborated with our metabolomic analysis, and demonstrated that somatic cells convert from an oxidative state to a glycolytic state in pluripotency. Interestingly, the bioenergetics of various somatic cells correlated with their reprogramming efficiencies. We further identified metabolites that differ between iPSCs and ESCs, which rev

SUBMITTER: Panopoulos AD 

PROVIDER: S-EPMC3252494 | biostudies-literature | 2012 Jan

REPOSITORIES: biostudies-literature