Expression patterns of non-coding spliced transcripts from human endogenous retrovirus HERV-H elements in colon cancer.

ABSTRACT:

Background

Up-regulation of the most abundant H family human endogenous retrovirus (HERV-H), especially env-related transcripts, correlates with colon cancer. However, expression pattern of spliced non-coding transcripts of HERV-H is not clear.

Methodology/principal findings

In this study, expression of HERV-H spliced transcripts in colon cancer was investigated by a RT-PCR strategy using primers targeting the tRNA(His) primer-binding site and the R region in the 3' long terminal repeat (LTR), followed by cloning and sequencing of the amplicons. Sequences were then assigned to individual HERV-H loci by employing private nucleotide differences between loci. Different expression patterns of HERV-H spliced transcripts from distinct active elements were found in colon cancer cel

SUBMITTER: Liang Q 

PROVIDER: S-EPMC3253121 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature