Unique TGFBI protein in lattice corneal dystrophy.

ABSTRACT:

Purpose

Specific components of transforming growth factor-beta-induced protein (TGFBIp) responsible for amyloid deposits in lattice corneal dystrophy (LCD) have not been delineated. LCD has been associated with various TGFBIp mutations such as R124C, L518P, and L527R. Using recombinant TGFBIp, this study was undertaken to identify TGFBIp components potentially contributing to the protein deposits in LCD.

Methods

Recombinant wild-type (WT) TGFBIp and four mutants (R124C, R124H, L518P, and L527R) were generated in HEK293FT cells. WT and mutant TGFBIp were collected from crude cell lysates or purified from culture media. Immunoblot analyses were performed with four different anti-TGFBIp antibodies raised against various regions of TGFBIp.

Results

Consistent with the auth

SUBMITTER: Han YP 

PROVIDER: S-EPMC3253538 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature