Hydride transfer versus hydrogen radical transfer in thymidylate synthase.

ABSTRACT: The nature of a H-transfer in the thymidylate synthase catalyzed reaction was investigated by comparison of the wild-type enzyme with the W80M mutant. The nature of the H-transfer was not affected, as indicated by intrinsic isotope effects and their temperature dependence. These findings support a single-step hydride transfer instead of a two-step radical transfer.

SUBMITTER: Hong B 

PROVIDER: S-EPMC3253763 | biostudies-literature | 2006 May

REPOSITORIES: biostudies-literature