A quantitative proteomic approach for detecting protein profiles of activated human myeloid dendritic cells.

ABSTRACT: Dendritic cells (DC) direct the magnitude, polarity and effector function of the adaptive immune response. DC express toll-like receptors (TLR), antigen capturing and processing machinery, and costimulatory molecules, which facilitate innate sensing and T cell activation. Once activated, DC can efficiently migrate to lymphoid tissue and prime T cell responses. Therefore, DC play an integral role as mediators of the immune response to multiple pathogens. Elucidating the molecular mechanisms involved in DC activation is therefore central in gaining an understanding of host response to infection. Unfortunately, technical constraints have limited system-wide 'omic' analysis of human DC subsets collected ex vivo. Here we have applied novel proteomic approaches to human myeloid dendritic cells (

SUBMITTER: Schlatzer DM 

PROVIDER: S-EPMC3253886 | biostudies-literature | 2012 Jan

REPOSITORIES: biostudies-literature