Identification of the heparin binding site on adeno-associated virus serotype 3B (AAV-3B).

ABSTRACT: Adeno-associated virus is a promising vector for gene therapy. In the current study, the binding site on AAV serotype 3B for the heparan sulfate proteoglycan (HSPG) receptor has been characterized. X-ray diffraction identified a disaccharide binding site at the most positively charged region on the virus surface. The contributions of basic amino acids at this and other sites were characterized using site-directed mutagenesis. Both heparin and cell binding are correlated to positive charge at the disaccharide binding site, and transduction is significantly decreased in AAV-3B vectors mutated at this site to reduce heparin binding. While the receptor attachment sites of AAV-3B and AAV-2 are both in the general vicinity of the viral spikes, the exact amino acids that participate in electrosta

SUBMITTER: Lerch TF 

PROVIDER: S-EPMC3253896 | biostudies-literature | 2012 Feb

REPOSITORIES: biostudies-literature