AMP-activated protein kinase regulates E3 ligases in rodent heart.

ABSTRACT:

Rationale

The degradation of proteins by the ubiquitin proteasome system (UPS) is required for the maintenance of cellular homeostasis in the heart. An important regulator of metabolic homeostasis is AMP-activated protein kinase (AMPK). AMPK activation inhibits protein synthesis and activates autophagy, but whether AMPK plays a role in regulating protein breakdown through the UPS in the heart is not known.

Objective

To determine whether AMPK enhances UPS-mediated protein degradation by directly regulating the ubiquitin ligases Atrogin-1 and muscle RING finger protein 1 (MuRF1) in the heart.

Methods and results

Nutrient deprivation and pharmacological or genetic activation of AMPK increased mRNA expression and protein levels of Atrogin-1 and MuRF1 and consequently enha

SUBMITTER: Baskin KK 

PROVIDER: S-EPMC3254015 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature