Variability in urinary oxalate measurements between six international laboratories.

ABSTRACT:

Background

Hyperoxaluria is a major risk factor for kidney stone formation. Although urinary oxalate measurement is part of all basic stone risk assessment, there is no standardized method for this measurement.

Methods

Urine samples from 24-h urine collection covering a broad range of oxalate concentrations were aliquoted and sent, in duplicates, to six blinded international laboratories for oxalate, sodium and creatinine measurement. In a second set of experiments, ten pairs of native urine and urine spiked with 10 mg/L of oxalate were sent for oxalate measurement. Three laboratories used a commercially available oxalate oxidase kit, two laboratories used a high-performance liquid chromatography (HPLC)-based method and one laboratory used both methods.

Results

Intra-

SUBMITTER: Maalouf NM 

PROVIDER: S-EPMC3254161 | biostudies-literature | 2011 Dec

REPOSITORIES: biostudies-literature