Bacterial whole-cell biosensor for glutamine with applications for quantifying and visualizing glutamine in plants.

ABSTRACT: A whole-cell biosensor for glutamine (GlnLux) was constructed by transforming an Escherichia coli glutamine (Gln) auxotroph with a constitutive lux reporter gene. Measurements of Gln in plant extracts using GlnLux correlated with quantification using high-performance liquid chromatography (Spearman's r = 0.95). GlnLux permitted charge-coupled-device (CCD) imaging of Gln from whole plant organs.

SUBMITTER: Tessaro MJ 

PROVIDER: S-EPMC3255742 | biostudies-literature | 2012 Jan

REPOSITORIES: biostudies-literature